Biokimia Analitik

Tahun Akademik:
Genap 2022/2023
Kelas-Offr:
G-G
Deskripsi:
Prinsip dasar aplikasi spektroskopi, mikroskopi, kromatografi, pengendapan, dialisis dan elektodialisis, elektroforesis, sentrifugasi (general dan gradien), PCR dan Real Time PCR, omics, LC-MS-MS, immune-assay untuk karakterisasi protein, DNA, RNA, dan lipida.
Capaian Pembelajaran
  • Memahami prinsip dasar karakterisasasi biomolekul, identifikasi dan kuantisasi biomolekul.
Daftar Pustaka:
  • Skook D.H.,West, D. M., Holler, J. F, and Crouch, S. R., 2013 Fundamental of Analytical Chemistry. 9thed. Cengage Learning US.
  • Simon de Vries, Suharti, Laurice AM Pouvreau. 2007. Nitric oxide reductase: structural variations and catalytic mechanism (in Biology of the nitrogen cycle). Elsevier.p. 57-66.
  • Suharti Suharti, Katsuhiko S Murakami, Simon De Vries, James G Ferry. 2008. Structural and Biochemical Characterization of Flavoredoxin from the Archaeon Methanosarcina acetivorans,. Biochemistry, Vol. 47. Issue. 44. P. 11528-11535.
  • Suharti Suharti, Mingyu Wang, Simon De Vries, James G Ferry. 2014. Characterization of the RnfB and RnfG subunits of the Rnf complex from the archaeon Methanosarcina acetivorans. PLoS One. Vol.9. issue 5. e97966
  • Holme, D.J and Peck H, 1998. Analytical Biochemistry. 3th ed. Prentice Hall.
  • Katoch, R, 2011. Analyticak Technique in Biochemistry and Molecular Biology, Springer.
  • Mikkelsen S. and Corton E. 2004. Bioanalytical Chemistry., John Willey & Son. Inc Publication.
  • Gault V. A., and McClenaghan, N. H., 2009. Understanding Bioanalytical Chemistry, John Willey & Son. Inc Publication.
  • Suharti, Marc JF Strampraad, Imke Schröder, Simon de Vries. 2001. A Novel Copper A Containing Menaquinol NO Reductase from Bacillus azotoformans, Biochemistry, Vol: 40, issue :8. p. 2632-2639
  • Suharti,, Hendrik A Heering, Simon de Vries, 2004. NO reductase from Bacillus azotoformans is a bifunctional enzyme accepting electrons from menaquinol and a specific endogenous membrane-bound cytochrome c 551, Biochemistry, Vol. 43, issue42. P. 13487-13495
  • Suharti, dan Simon de Vries. 2005. Membrane-bound denitrification in the Gram-positive bacterium Bacillus azotoformans. Biochemical Society Transactions, Vol. 33. Issue. 1. p. 130-133
  • Shen Lu, Suharti,, Simon de Vries, Pierre Moënne-Loccoz. Two CO Molecules Can Bind Concomitantly at the Diiron Site of NO Reductase from Bacillus azotoformans. Journal of the American Chemical Society, Vol. 126. Issue 47. P. 15332-15333.